FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9233774
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG48250.062.8861475
GGTATAG132500.062.6515735
GTTATAG153850.062.396685
CGTATAA64700.062.3133935
CGTATAG72500.061.0157935
CGATAAG32400.060.7056
CGTTATA84900.060.5133934
ATTATAG174200.060.1501665
GTTATAA123850.059.623795
TTATAAC64850.059.578876
CTATAGG85150.059.554826
TTATAGC103650.059.4597666
GGATAGT95750.059.248516
TTATACG78800.059.20216
TATAGCG73450.059.176767
GGGATAG111350.058.9624865
GCGGATA57700.058.9554064
TATACGC38250.058.9217727
TTATAGT180800.058.8835456
CTATACG38750.058.795286
Produced by FastQC (version 0.11.5)