FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3613540
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG63600.060.5825585
GGATAGT43550.060.509596
CGGATAG20100.059.8935135
CGTTATA32750.059.6266334
CTATACG13150.059.346696
GGTATAG53250.058.885155
CGTATAA23800.058.8166665
CGATAAG14400.058.8125846
TATAACG17750.058.3585857
CGTATAG28200.057.9542355
TATAGGG75450.057.9314737
CGGATAA18250.057.7192965
GATAGTC15850.057.626557
TTATAGT78000.057.608666
TTATACG29900.057.5852556
TTTATAG82250.057.5677535
TATAGCG27250.057.5337757
TTATAAC24050.057.1866766
ATTATAG68450.056.852395
GTTATAA43050.056.822645
Produced by FastQC (version 0.11.5)