FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085524
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG56050.065.061545
CGTATAG27500.064.903745
GGTATAG47550.064.621385
CGTATAA28000.064.6196755
CTATAGG26650.064.4787456
CGGATAG16050.063.234665
CCTATAG17950.062.975315
TATAGCG26000.062.455387
ATTATAG51750.061.9465875
TTTATAG58250.061.883315
TTATAGC30450.061.719056
TTATAGT62800.061.6907776
CGTTATA34400.061.6518944
TATAACG17750.061.3178947
GGGTATA48300.061.2992974
TATAGGC29400.060.8273357
GTATAAG39300.060.821966
CATATAG33550.060.6060375
TTATACG26600.060.5213286
TTATAGA41450.060.4533966
Produced by FastQC (version 0.11.5)