FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3816364
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG19500.063.177495
GTTATAG64950.062.345995
GGATAGT47000.060.61516
CGTTATA32250.059.7965624
CGTATAA23900.059.4541855
GGTATAG58650.059.1967625
TTTATAG92000.059.11775
CTATACG14950.058.9947936
CGTATAG28200.058.9520345
TTATAGC42200.058.6355866
TATAGCG27950.058.601267
TTATAGT86300.058.520746
TATAGGG81250.058.408937
GTATAAG44150.058.1861536
ATTATAG67950.057.8421755
GGTTATA66000.057.483034
GTATAGG68200.057.0656476
TATAACG17000.057.0261157
TTATAAC23700.056.8547446
GGGTATA55600.056.652884
Produced by FastQC (version 0.11.5)