FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3412478
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG18900.065.920865
CGTATAA22600.064.88445
TATAACG19150.064.512967
GTTATAG70800.062.7776645
GGTATAG64500.062.127015
CTATACG14600.061.8454676
TTATATG88700.060.8417636
ATTATAG59500.060.7600365
CGGATAA15050.060.6930165
CGCGATA12400.060.6808244
CGTTATA34700.060.6149444
GTATAAG45750.060.35696
TTATAGT75900.060.266356
CGATAAG12050.060.120746
GGGATAG52400.060.109895
GGTTATA68800.060.0753334
TTTATAG77900.060.0210655
CGTATAG31150.059.995395
ATAGGGG47200.059.98488
CGCTATA18750.059.9153984
Produced by FastQC (version 0.11.5)