FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS3-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS3-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5253228
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG23400.062.9660725
CGTATAG35900.062.5865445
GTTATAG79350.061.5713735
GGTATAG70850.060.8570945
CGTATAA35050.060.3099865
CTATACG20300.060.168586
CGTTATA45150.060.0736924
TATAGCG40950.059.5682877
GGGTATA66100.059.0885164
GGGATAG57950.059.0045
CGATAAG16450.058.7196586
TTATACG37050.058.6602256
TTATAAC33050.058.5590486
GATAGGT44600.058.538317
GGATAGT51550.058.4540826
GGTTATA78950.058.071034
TTATAGC51350.058.068356
TATAACG26600.058.022067
CTATAGG43150.057.9916656
GTATAGG82000.057.959726
Produced by FastQC (version 0.11.5)