FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15518264
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG76050.064.1051945
CGTATAG110700.062.313245
GTTATAG249650.062.061075
CGTATAA107150.061.3074725
GGTATAG221250.061.2007525
TATAGGG272700.060.7158557
TTATAGC170000.060.4023556
CTATAGG137050.060.08776
ATTATAG276900.059.3535165
TATAACG84500.059.3504187
CTATACG65550.059.264286
CGTTATA139500.059.1576424
GTTATAA198550.059.1376345
GGATAGT155200.059.059056
TATAGCG134700.059.0043267
CGATAAG54300.058.587746
TATACGC68900.058.5658387
TTATAAC107850.058.216356
TATAGGC176950.058.1475037
GTATAAG185000.058.0587736
Produced by FastQC (version 0.11.5)