FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16507416
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG117300.063.996495
CGGATAG74550.063.6095
CGTATAA124350.063.0700155
GTTATAG250750.062.3729485
TTATAGC171350.061.3337366
GGTATAG201450.061.3245435
TTATAAC112750.061.1784176
CTATACG68400.060.5792356
TATAGGG276100.060.422187
TATAACG91950.060.3247077
ATTATAG294150.060.1540835
CGTTATA149600.060.0511254
CGATACG21750.059.8565756
CTATAGG142350.059.717036
TATAGCG141500.059.653867
GGATAGT143600.059.4896666
TATACGC70550.059.3269237
GTATAAG188000.059.066396
TATAAGC189900.058.9347087
TATAGGC171250.058.752317
Produced by FastQC (version 0.11.5)