FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9542616
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG44250.061.056615
GGTATAG112100.061.033715
GTTATAG142700.060.7478185
CTATACG40150.060.2311486
CGTATAA70200.059.9731945
TTATAGC102500.059.784826
CGATACG11900.059.7004626
GTATAAG103650.059.4929166
TATAGCG76600.059.3928457
TTATAAC69150.059.3655436
CGTATAG66000.059.2824975
ATTATAG173000.058.9484065
GTATAGG138600.058.8077936
CGTTATA88550.058.7298474
TATAGGG158450.058.639717
TATACGC41250.058.6237567
TATAGGC104300.058.584067
GGATAGT78900.058.3723956
CGATAAG34400.058.2941326
CTATAGG87400.058.2212646
Produced by FastQC (version 0.11.5)