FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8763755
Sequences flagged as poor quality0
Sequence length76
%GC42

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA61550.063.2309575
GTTATAG168900.062.5999685
GGATAGT109550.062.3301126
TATAACG41100.062.2463267
CGGATAG53450.062.009085
CTATACG34100.061.7867166
TATAGGG205800.061.7814677
GGTATAG126850.061.4720575
CGTTATA84700.061.196234
TTTATAG254850.061.1397365
GTTATAA111150.060.9605565
ATTATAG182150.060.87085
TTATAGC104150.060.6556136
TTATAGT225400.060.2929426
TATACGC29950.060.17957
GTATAAG103750.059.7087826
TTATATG215950.059.673796
CGATAAG31750.059.4152686
GTATAGG165400.059.1636126
CGTATAG80850.059.045585
Produced by FastQC (version 0.11.5)