FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7183520
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG120750.061.677245
TATAACG39100.061.1325237
GGTATAG102400.061.1093985
CGTTATA58500.060.7226374
CGGATAG37500.060.5694855
TTATAGC73200.060.5289466
CGTATAA49650.059.9156275
GTTATAA93000.059.910175
CTATACG29800.059.778086
TATAGCG57600.059.7254457
GGATAGT78650.059.6719866
TATAGGG141750.059.2538457
ATTATAG140500.058.711575
CGGATAA32850.058.702515
TATACGC30650.058.5753977
GTATAGG120100.058.339346
TTATAGG159250.058.3039866
TTTATAG145700.058.2975965
CTATAGG62500.057.9563146
GTATAAG92500.057.699046
Produced by FastQC (version 0.11.5)