FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7966219
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG41300.064.4879465
CGTATAA52000.063.4676325
GTTATAG137300.063.3176775
GGTATAG114250.063.011825
TTATAGC83900.062.6127366
CTATAGG76300.062.014916
CGTTATA70950.061.6597184
GGATAGT76700.061.417716
ATTATAG149900.061.380855
GTTATAA96900.061.2556275
CGATAAG27150.061.2304846
CGTATAG64300.061.0152785
TATACGC31850.060.9844787
GTATAAG94250.060.9356086
TTTATAG171200.060.571995
TTATAAC51350.059.8408856
TATAGGG157500.059.8177767
TATAAGC95600.059.8177347
GGTTATA127750.059.750114
CTATACG30900.059.6892436
Produced by FastQC (version 0.11.5)