FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10381185
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG140650.062.5051575
CGATAAG34200.062.012966
CGGATAG52050.061.926265
GTTATAG166250.061.553355
TTATAGC105750.061.423366
CGTATAG77650.061.2511065
CGTATAA69050.061.175795
GGATAGT100900.060.5951276
TTTATAG212500.060.4096575
CGTTATA92800.060.2649424
CTATACG42000.059.912246
CTATAGG88950.059.8438076
ATTATAG186700.059.6661835
TTATAAC64950.059.6491666
CGATACG12750.059.5642246
GTATAAG119450.058.890586
GTATAGG174500.058.6632846
TATAGGG188450.058.608717
TATAAGC117300.058.4463927
GTTATAA131500.058.4443475
Produced by FastQC (version 0.11.5)