FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4033547
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG19800.063.8080145
GGTATAG55400.063.4876865
GTTATAG65850.062.3943825
CGTATAA29800.062.3607675
TTATAGC42600.061.6969266
CGATAAG13700.060.7980426
GGATAGT37250.060.7870676
ATTATAG72500.060.7744335
CGTTATA37250.060.6931154
TATAACG21350.060.322287
CGTATAG29600.060.2992135
TATACGC16150.060.02547
TTTATAG82250.059.995195
CTATACG18150.059.967646
GTATAGA51950.059.6872256
TATAGTC37550.059.5550657
GTATAAG46450.059.2955676
GTATAGG66050.059.132276
TTATAGT73850.058.8104486
TATAGGC45050.058.7292987
Produced by FastQC (version 0.11.5)