FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3287017
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATAG49700.063.374965
GTTATAG56550.062.9390145
CGTATAA24150.062.458535
CTATAGG30800.062.3820846
CGATAAG11550.062.116956
TTATAAC22250.061.973276
CGGATAG18700.061.5723765
CGTTATA31300.061.1610034
TATAAGC41950.061.1500787
TATAGGG62400.061.0756767
ATTATAG63100.060.9536865
GTATAAG37550.060.8613326
CTTATAA29100.060.8545
CTATACG14250.060.66256
GTATAGG58000.060.6423876
GGATAGT31550.060.4554376
TATAGGC39700.060.384447
GTTATAA42400.060.3369185
GTATAGA44000.060.2117736
TTATAGC35550.060.052136
Produced by FastQC (version 0.11.5)