FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4432595
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG21700.062.4130975
GGTATAG62600.062.3899085
GTTATAG73600.062.24245
CGTATAA34650.062.0139855
GTTATAA58950.061.0880245
CGATACG6650.061.0465166
CTATACG19400.060.973276
TTATAGC44300.060.908126
CTATAGG39450.060.5009046
GTATAAG54850.060.2947776
ATTATAG75800.060.2050445
CGATAAG13550.060.178476
GGATAGT49900.060.10426
TTTATAG93300.059.4527785
CGTTATA41450.059.354734
CTTATAA42800.059.1996735
TTATAGT92400.059.0092356
TTATAAC30750.058.9534386
CGGATAA19500.058.6864245
TATAGCG37150.058.593737
Produced by FastQC (version 0.11.5)