FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6547472
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG37650.064.1376345
GTTATAG125400.063.714355
GGTATAG112100.062.750815
CGGATAA27150.062.1306765
TTATAGC68250.061.5329136
CTATAGG59450.061.2223976
CGTTATA69300.061.2065934
TATAACG34450.061.1541827
CTTATAA55000.061.08545
GTTATAA91650.061.0201345
CGTATAA49650.060.9008525
TTATAGT142650.060.7691426
GGATAGT78200.060.7298136
ATTATAG116550.060.6551865
TTTATAG153200.060.5825
CGATAAG19000.059.678836
ATAGGCG27350.059.242858
GCGGATA40200.059.1115844
GTATAAG85100.059.0134746
CGATACG8150.058.8290446
Produced by FastQC (version 0.11.5)