FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794287
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG50400.063.8156935
CGGATAG15650.062.6161235
GGTATAG44950.062.5992135
CGATAAG8050.062.170266
CGTATAA21450.062.1641735
TTATAGC29650.060.9070436
TTATAGT56950.060.839276
GTTATAA34650.060.8045125
CTATAGG23500.060.7623836
ATTATAG48600.060.6342935
TTTATAG62500.060.3644525
GTATAAG33500.059.9666256
CGTATAG23150.059.866895
CTATACG14150.059.855146
GGATAGT32900.059.5709656
CGGATAA14400.059.545115
CCGATAA6950.059.4209675
CTTATAA24150.059.4167985
CGTTATA27600.059.217534
TATAGCG22350.059.190097
Produced by FastQC (version 0.11.5)