FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800241
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG35200.063.4318125
CGTTATA19400.061.1543124
TATAACG9500.061.150717
CGTATAA14200.061.121285
CGGATAG9300.060.5859685
TTATAAC11850.060.5430956
GTTATAA23900.060.4757465
CTATAAA23500.060.1648186
GATAGTC7750.060.0574657
GGATAGT22450.060.016896
TTATAGC18850.059.9680986
TTATAGT44900.059.9389466
CGTATAG14200.059.8889965
TTATAGG48300.059.1975866
TATAGGG41200.059.119277
ATTATAG33900.059.0507555
TTATATC22850.058.8131036
GGTATAG28100.058.535685
CCGATAA3600.058.3281065
CTATACG7400.058.1704566
Produced by FastQC (version 0.11.5)