FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349672
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAA17600.063.4358565
GTTATAG23750.062.777325
GGTATAG21250.062.421915
TTATAGC15200.062.3996966
CGGATAG7050.062.0551265
CCGATAC1300.061.921475
TTATAAC9850.061.4704866
CGTATAA10900.061.3286825
CGTATAG11600.061.248415
TTTATAG29250.061.143715
ATTATAG24050.060.830025
TTATAGT25950.060.826946
TATAGGG28100.060.6567887
ATAGGGG15400.060.452988
TATAACG6200.060.401667
CGTTATA14000.060.2484364
CTATAGG12750.060.1160856
GGGTATA21150.060.0693634
TTATAAG19900.059.973326
TATAGCG10600.059.76267
Produced by FastQC (version 0.11.5)