FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37326507
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG188450.063.7558675
GGTATAG555950.062.6116645
GTTATAG648000.062.483215
CGTATAA289050.061.8593645
CGTATAG293450.061.5043075
CTATAGG323350.060.016746
TTATAGC411150.059.8750346
GTTATAA510350.059.7505845
GGATAGT394400.059.702536
TATAGGG697900.059.6543547
CTATACG160850.059.443366
ATTATAG695050.059.2152865
CGTTATA369050.059.1375664
TATAACG203300.059.1321647
TATAGCG298150.059.019417
TTATAGT792950.058.7324376
TTTATAG813800.058.7241255
TTATAAC264650.058.702546
TTATAAG546200.058.680346
CGATAAG114750.058.2843136