FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17631261
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG336650.063.622915
CGGATAG100350.063.369215
CGTATAG151650.062.079845
GTATAAG218200.061.5428736
GGTATAG307000.061.5368465
CGTATAA134100.061.3309675
GTTATAA236600.061.2831235
TTATAGC199000.060.9559636
ATTATAG318400.060.6635865
GGATAGT214850.060.6454736
TTATAGT391200.060.2978446
CTATAGG159250.060.2379726
TATACGC74900.060.2290047
CGATACG23550.060.1872946
CGGATAA79850.060.002495
TATAGGG366450.059.9953777
TTTATAG404750.059.8097085
CGTTATA181650.059.8035624
CTATACG77550.059.660986
TATAGCG154650.059.1095777
Produced by FastQC (version 0.11.5)