FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18074668
Sequences flagged as poor quality0
Sequence length76
%GC37

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA135100.062.6657265
GGTATAG222900.062.3975265
CGTATAG131050.062.225515
GTTATAG274400.061.9233865
CTATACG76050.061.7135246
CGGATAG84100.061.2994165
TTATAGC187700.061.1776436
CTATAGG155300.061.1630556
GTATAAG194200.060.806036
ATTATAG317300.060.687625
TATAGGG294900.060.312137
TTATAAC124800.060.0697336
TATACGC76750.059.9186137
CGTTATA164750.059.8426784
TATAGCG153150.059.6213577
GTTATAA243450.059.50255
GGATAGT162750.059.309476
TATAAGC208600.059.275357
TTTATAG346700.059.1754465
CCTATAC64250.059.1570175