FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15923527
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG76750.062.6532485
CGTATAG108650.061.909735
GTTATAG248400.061.5130655
TTATAGC174200.061.396056
CGTATAA113200.061.307215
GGTATAG204650.060.9995465
GTATAAG172700.060.95666
GTTATAA201600.060.568345
ATTATAG296250.060.4966935
TTATAAC108900.060.4821136
CTATACG64700.060.1500136
TATAGGG276100.060.092997
CTATAGG141100.059.7013746
CGTTATA141100.059.6269654
GGATAGT140050.059.424296
TATAACG88250.059.1266337
TATAAGC196400.059.0693667
CGATAAG59600.059.0146
TATAGCG133850.058.8018957
TTTATAG311850.058.7722175
Produced by FastQC (version 0.11.5)