FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS2-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS2-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17254353
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG96150.063.881435
GTTATAG317250.062.7928355
CGTATAG132100.062.1809125
TATAGGG373850.061.7191477
GGATAGT215300.061.511166
TATAACG71250.061.4478457
CGTATAA109650.061.442515
CGTTATA160350.060.7641034
GGTATAG260300.060.7596135
ATTATAG345100.060.3317385
CGATACG26000.060.3047456
GTTATAA213450.060.158695
CTATACG63050.059.7829676
TTATAGC191700.059.7362566
TTTATAG496600.059.227885
GTATAGG317000.059.0554776
TATAGGC192650.059.0403027
TTATATG426100.059.0395976
GTATAAG193350.058.936876
CTATAGG165800.058.8722766
Produced by FastQC (version 0.11.5)