FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS1-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS1-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2062329
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTATAG34350.062.352885
GTTATAA27050.060.9376075
CGTATAG13000.060.8410765
TTATAGT45450.060.8310056
GGATAGT25150.060.670886
CGTATAA17300.060.4862825
TCTATAA17650.060.278255
TTTATAA32350.060.149545
GGTTATA33000.060.1313444
ATAGGGC9800.059.9935348
TTATAGG41650.059.490836
TTTATAG45750.059.284675
CGGATAG10150.058.9605945
CGTTATA19650.058.9518244
CTATACG8950.058.654326
CGGATAA10850.058.382225
TTATAGC21000.058.328466
TTATAAC11750.058.0802576
TATAACG8200.058.043937
ATAGTCG8550.057.713088
Produced by FastQC (version 0.11.5)