FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS1-5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS1-5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908687
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG20490.10735128389306366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATACG2000.062.997226
GTTATAG28350.060.8615075
CGTATAG12100.060.741125
CGTTATA16650.059.9072654
TTATACG14250.059.435976
TTATAGG35300.058.8925826
GGTTATA28600.058.861044
ATAGGGC8100.058.7612958
CGTATAA16650.058.435865
TTATAGT40400.058.3885086
GCGTATA14550.058.2104844
TCTATAA17400.058.1296165
TTTATAG38850.057.9253735
TTATAGC19400.057.909816
ATTATAG32600.057.6508265
CGGATAA9800.057.497465
TATAGGG29450.057.3998457
CGGATAG8050.057.388775
GGTATAG26300.057.354885
CCGATAC2450.057.140335
Produced by FastQC (version 0.11.5)