FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS1-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS1-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3039995
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATCATATAATTTCTGTTGAATTACGTTAAGCATGTAATAATTAACATG49710.1635200057894832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG20900.062.6264465
GTTATAG48350.062.394065
CGGATAA15000.061.361635
CGATAAG11300.060.3932766
TCTATAA28650.060.2219355
TTATAGC31200.059.898936
GGATAGT28500.059.6793146
GTTATAA44300.059.4082225
TTATAGT59950.059.0777246
CTATAGG22350.059.033196
CGTTATA32750.058.880664
ATTATAG50200.058.770085
TATAGGG49700.058.7256477
TATAGCG23150.058.6541677
TTTATAG58300.058.528655
GGTTATA52700.058.505644
CGCTATA17650.058.493794
GCGTATA28600.058.2469754
ATAGGGC12800.058.234548
CCGATAC2950.058.1308255
Produced by FastQC (version 0.11.5)