FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS1-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS1-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3307737
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATAG31450.061.4242745
TCTATAA29900.059.5755775
GTATAGG43400.059.108376
GTTATAG55100.059.0680965
CGGATAG15600.059.0001075
GGATAGT35450.058.8389326
CGATAGT16450.058.5061536
CGTATAG20800.058.214935
CTATACG12300.057.75986
GGTTATA59850.057.5961844
CGTATAA25650.057.576695
CGTTATA33600.057.493854
CGGATAA15550.057.389375
TATAGGG49350.057.228797
GCGGATA17750.057.1769834
TTTATAG76450.057.0377165
TTATACG26200.057.0376136
GATAGGG25800.056.9715167
TATATCG31100.056.9401177
TATACGC12500.056.8347857
Produced by FastQC (version 0.11.5)