FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3890671
Sequences flagged as poor quality0
Sequence length76
%GC38

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG18250.060.596525
CGTATAG22550.060.2155535
GTTATAG58000.060.036945
TATACGC14700.059.2788737
GGGATAG42450.058.2861255
TATAACG15350.057.908627
TTATAGG65300.057.7199556
TATAGGG52200.057.6561787
GGATAGT39550.057.6047066
CGTTATA37650.057.351354
TTATACG29550.056.846966
TTTATAA57900.056.695395
GTATAGG49700.056.6843266
ATAGGGC15950.056.6078838
TATAGGT60900.056.6026577
TTTATAG75100.056.3856935
TCTATAA33750.056.3053365
CGCTATA17700.056.1524284
CGTATAA29500.056.1128855
TTATAGC38050.056.104626