FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-RS1-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-RS1-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089185
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCAATAAAGTTTCTTAAGATTGAATCCTGTTGCCGGTCTTGCGATGAT53850.1316888328603377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG28100.061.7737855
TTATAGG73650.061.630556
GTTATAG64000.061.189785
ATAGCGG11850.060.836168
GGGATAG46000.060.635835
TATAACG19550.060.3255587
GGATAGT44050.059.903786
TTATAAC29350.059.1428686
CGGATAG19950.058.7666175
CGTATAA35900.058.393055
GCGTATA34950.058.2784
TTATAGT92250.058.0814746
TCTATAA36150.057.989235
TTTATAG90000.057.939215
CGTTATA38700.057.8759084
TATAGGT70700.057.6666457
TATAGGG62450.057.663527
GTATAGG55150.057.6194576
GTATAAG44000.057.585716
CGGATAA20550.057.5617075
Produced by FastQC (version 0.11.5)