FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS90mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS90mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2254794
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG42250.064.859316
CTTATAG20200.064.4508745
TTATAGT50600.061.626066
CTATAGG15850.061.1628576
CGGATAG11300.061.013375
GTATAGG34950.060.6823926
TATAGTG50200.060.372867
GTTATAG42450.060.3490755
ATAGGGC10850.060.3156288
TATAGGG37500.060.0077447
CCTATAC7950.059.8699655
TATAGGT41350.059.3294227
CGATAGT10350.059.1745456
GGTATAG32300.059.159895
CGTATAG17050.059.116045
GCGATAA7650.059.015425
TTTATAG50400.058.606955
TATAGGC24100.058.0858737
GCGATAG10400.057.8805055
CGATAGG8900.057.8048866
Produced by FastQC (version 0.11.5)