FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS90mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS90mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4499662
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG84600.064.660136
CGATAGG16200.062.003316
GCGATAG20600.061.84185
TTATAGT105400.060.7656946
CTATAGG30450.060.571916
CGGATAG21150.060.39915
GTATAGG68650.060.3613746
GTTATAG82200.060.3317685
ATAGGGC17200.060.0236328
CTTATAG38750.059.8811075
GGTATAG63750.059.730585
TTTATAG103950.059.7278755
CCTATAC14600.059.4493065
CCGATAG9500.059.3130535
CGTATAG32500.058.9049725
TATAGGG73000.058.872037
TATAGTG101150.058.715057
CGATAGT22200.058.645946
CGTATAA29200.058.6103065
TATAGGT83600.058.524057
Produced by FastQC (version 0.11.5)