FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS90mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS90mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2374191
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAG11950.064.428215
TTATAGG45500.063.1470456
GTTATAG42400.062.233865
TTATAGT50500.061.815126
GTATAGG36850.061.16036
GCGATAG11050.060.807935
CTATAGG18300.060.6218876
CCTATAC8150.060.5456285
TATAGGG36850.060.4942447
GGTATAG34400.060.3278055
CGTATAA15850.060.2774165
TTTATAG51700.059.6357425
CGATAGT11600.059.131566
CTTATAG22500.058.9492075
GTTATAA32450.058.8842545
ATAGGGC9800.058.563898
GATAGGT19500.058.5052877
GGTTATA42650.058.258674
CGATAGG8050.058.2545976
TATAGTG54300.057.8102347
Produced by FastQC (version 0.11.5)