FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS90mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS90mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3101667
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG53900.065.1229256
TTATAGT69950.061.4373366
GCGATAG12700.061.1745765
GTTATAG55300.061.0694355
CGATAGG12100.060.7373286
TATAGGG48550.059.6846777
CTTATAG25750.059.663545
GTATAGG44350.059.4976046
GGTATAG40900.059.4679835
CGATAGT13700.059.2637566
CGTATAA20300.058.959235
TATAGGT53150.058.4697767
CTATAGG22950.058.403366
CGGATAG13200.058.3271145
ATTATAG49450.058.3153235
CCGATAG6450.058.0558245
TATAGTG65750.057.9633987
CGTATAG22400.057.962575
CCCGATA4600.057.8199234
TTTATAG68500.057.782165
Produced by FastQC (version 0.11.5)