FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS90mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS90mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2884215
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG53850.065.70456
GCGATAG13300.063.678585
CTTATAG27200.062.7885635
TTATAGT64400.062.6575056
GTTATAG51300.062.080285
CGGATAG12950.061.886425
CTATAGG22050.061.4231386
TATAGGT53500.060.0497257
CGATAGG12400.059.8334246
CGATAGT13200.059.6538166
TATAGGG47100.059.5159957
TTTATAG65700.059.5000655
TATACGG23300.059.178297
CGTATAA21900.059.1271935
GGTATAG41100.059.009385
CCTATAC11550.058.782685
CCGATAG6150.058.6127055
TATAGGC30000.058.560477
GTATAGG40400.058.559186
CGTATAG22950.058.5569155
Produced by FastQC (version 0.11.5)