FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS90mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS90mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2194159
Sequences flagged as poor quality0
Sequence length76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG40650.065.00016
CGGATAG9700.063.8600355
GCGATAA8350.062.4492725
CTTATAG19800.061.1559225
TTATAGT52000.061.0423936
GTATAGG33700.060.335636
GCGATAG9700.060.2521255
GTTATAG43400.060.075055
GGTATAG33100.059.6319125
CGATAGG9100.059.6098336
CCGATAG5700.059.555865
CTATAGG18950.059.466766
CGTATAG17400.059.3335575
TATACGG18000.059.3000377
TATAGTG49450.059.165377
TTTATAG51150.058.430535
TATAGGG40150.058.3133747
ATAGGCG7450.058.249618
TTATACG21750.058.247456
AGTATAG22500.058.172365
Produced by FastQC (version 0.11.5)