FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS120mS5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS120mS5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5454596
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG106350.062.6892136
CGGATAG26150.061.964745
GCGATAG26650.061.4587865
CTTATAG49000.061.352525
TTATAGT119900.061.20896
CGATAGG20400.060.9022756
GTTATAG102450.060.5322885
GTATAGG84800.060.1310846
GGTATAG77950.059.398995
CTATAGG41850.059.2070126
TATAGGG91900.059.0630267
ATTATAG89200.058.7343675
TATAGGT100650.058.6918877
CGATAGT26800.058.2418826
CCTATAC18200.057.4956675
CGTATAG44700.057.2328265
TTTATAG123400.057.090525
CGTATAA39050.057.089175
TATAGTG116500.056.9549987
GGTTATA97400.056.5203824
Produced by FastQC (version 0.11.5)