FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS120mS3_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS120mS3_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2922621
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG51750.063.504566
CGGATAG12850.063.187995
GCGATAG12250.062.5687835
TTATAGT63250.061.8629956
GTATAGG45600.061.861426
GTTATAG50600.061.835335
CTATAGG21450.061.0230646
CTTATAG24850.060.7016565
TATAGGG46900.060.6659627
CGATAGT13150.060.1495486
CGTATAA21700.059.9974635
TATAGGT53650.059.752097
ATAGGGC12150.059.3349958
TATAGGC29500.059.0792167
GGTATAG42050.058.9273575
TTTATAG63800.058.6417165
GCGATAA10450.058.6099625
CGTATAG21050.058.3585745
GGTTATA53750.058.0161484
TATAGTG63400.057.8494727
Produced by FastQC (version 0.11.5)