FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS120mS3_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS120mS3_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5481196
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG97000.063.029986
GCGATAG26250.062.393965
CTATAGG40600.061.4595686
CTTATAG46700.060.9255755
GGTATAG79350.060.643155
TTATAGT120100.060.6102876
GTATAGG84800.060.0885166
CGGATAG25000.060.0541885
GTTATAG95350.059.9732135
CGATAGG19950.059.4679266
TATAGGT99850.058.7764057
CGATAGT28600.058.613216
CGTATAA36950.058.248765
GATAGGT45650.057.956097
GGGATAG54200.057.7896045
TATAGTG117950.057.6788187
CGTATAG42900.057.593495
TTTATAG120950.057.261925
TTATACG47700.056.933696
TATAGGG89550.056.8220527
Produced by FastQC (version 0.11.5)