FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS120mS2_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS120mS2_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9238740
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG170400.064.490896
CGGATAG39900.063.3289955
GCGATAG43850.062.4928745
CTTATAG80000.062.0775
TTATAGT197350.061.2879266
GTTATAG162900.061.036345
CGTATAG65850.060.853845
GTATAGG135250.060.7573976
CGATAGG36700.060.2683536
CTATAGG67800.060.032776
TATAGGG149200.059.860887
CGATAGT43500.059.4557046
CCTATAC34150.059.4395645
GGTATAG125750.059.3915635
CGTATAA63750.059.290065
ATTATAG147900.059.1102265
TATAGTG191400.058.9135067
TATAGGT172150.058.8129167
GATAGGT74800.058.7182927
TTTATAG203700.058.672965
Produced by FastQC (version 0.11.5)