FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS120mS2_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS120mS2_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5364358
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG100900.064.167856
CGGATAG24500.062.4241035
TTATAGT120600.061.289146
GTTATAG98800.060.8559655
CTTATAG49050.060.505355
CGATAGT25250.060.154116
GTATAGG80900.059.915366
GCGATAG24900.059.7346765
CTATAGG42450.059.689486
TATAGGG88000.059.6537067
TATAGGT105000.059.4279677
CGTATAG38500.059.2684825
CCGATAG11550.059.0866745
CGATAGG21200.058.934466
TTATACG47900.058.8893136
GGTATAG75450.058.8625835
CGTATAA36700.058.6470265
TTTATAG118700.058.437265
CCTATAC18950.057.9905665
GCGATAA19850.057.65335
Produced by FastQC (version 0.11.5)