FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS120mS1_noN.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS120mS1_noN.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3168523
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG69250.064.789276
CGGATAG15200.063.778025
CTATAGG25700.063.4581876
GTATAGG54200.062.8273056
TTATAGT78450.062.56
GGTATAG50400.062.2174645
GTTATAG62150.061.9984745
GCGATAG16600.061.3507355
CTTATAG29950.061.347575
TATAGGG58850.061.0743457
TTTATAG79400.060.9147455
TATAGGT69900.060.882337
CGTATAG25900.060.535915
ATAGGGC12900.060.4992528
TATAGTG77600.060.117837
CGATAGG13800.059.8504946
GGATAGT40650.059.2328646
CGATAGT17200.059.210596
CGTTATA35750.058.834664
GATAGGT28150.058.6811337
Produced by FastQC (version 0.11.5)