FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS120mS1_N.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS120mS1_N.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4219309
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG79000.063.7451676
CCGATAG8550.061.3958055
CGATAGG15150.061.2134446
GTATAGG60600.060.92476
GTTATAG77200.060.879675
CTTATAG37450.060.3662575
GCGATAG17400.060.337265
TTATAGT94500.060.214676
CGGATAG18950.060.2035335
CGTATAG30050.059.9758385
GGTATAG58300.059.7866675
TATAGGT75100.059.7853977
TATAGGG69850.059.4191867
CTATAGG31850.059.3332146
GCGATAA16450.058.9287765
TATAGTG91200.058.3253257
CGATAGT19950.057.8874746
TTTATAG95350.057.879475
ATAGGTG42150.057.7012678
CCTATAC15400.057.492795
Produced by FastQC (version 0.11.5)