FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS0mS3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS0mS3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3603301
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG72450.065.306956
TTATAGT90900.062.7936
CTATAGG29550.062.6497466
CGGATAG18600.062.466375
GTATAGG59250.062.1367456
GTTATAG75350.062.096815
CGATAGT16850.061.4770366
GGTATAG57800.061.3342065
CGTATAA22550.061.301585
TTTATAG88100.061.253315
CGTATAG29250.061.1386955
CTTATAG35050.060.9064035
ATAGGGC16750.060.79948
GCGATAG18050.060.6859635
TATAGGT70800.060.353657
TATAGGG63700.060.323157
TATACGG33050.060.250687
TATAGTG87550.059.9992267
CCGATAG7850.059.2929575
TATAGGC40100.058.996127
Produced by FastQC (version 0.11.5)