FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS0mS2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS0mS2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3471387
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG65250.065.061996
CGGATAG15800.062.4644365
CTATAGG25950.062.3091246
CGATAGG13800.061.6274576
TTATAGT75250.061.6249286
CTTATAG28850.061.01875
GCGATAG15350.060.8756565
GTATAGG52200.060.543056
GTTATAG65750.060.4676865
CGTATAA26400.060.1856165
GGTATAG51500.059.937995
TATAGGG57450.059.4558147
TATAGGT66100.059.352467
TTTATAG78300.059.2236145
ATAGGTG34350.058.6854258
TATAGTG75800.058.636647
CGATAGT16650.058.435616
CGTATAG26700.057.936445
TATAGGC36850.057.5532267
TTATACG29800.057.430116
Produced by FastQC (version 0.11.5)