FastQCFastQC Report
Tue 11 Aug 2020
H2KCKBGXG_n01_June2020-MTS0mS1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH2KCKBGXG_n01_June2020-MTS0mS1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899291
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATAGG77900.065.593386
CGGATAG21550.064.312085
TTATAGT98950.062.8869636
CTATAGG31750.062.8312646
GTATAGG65450.062.616956
GTTATAG79400.062.2384575
TATAGGG70900.062.046467
CTTATAG37850.061.9517445
CGTATAG32200.061.5184255
GCGATAG18400.060.4858745
CGATAGG14400.060.2745326
TTTATAG94550.060.1131065
TATAGGT82200.060.0309527
GGTATAG62200.059.980695
CGTATAA25750.059.938525
TATAGGC40600.059.0462767
TATACGG31200.058.8887947
CCTATAC12600.058.8857155
ATTATAG64550.058.4476625
TATAGTG96950.058.4422577
Produced by FastQC (version 0.11.5)