FastQCFastQC Report
Wed 25 Feb 2015
lane2_Undetermined_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71364162
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT1290960.18089752108348164Illumina Single End PCR Primer 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC215800.033.30087743
TGGTCGC227950.031.64407342
GTAGGGA244100.031.43939818
GTGGTCG237050.031.01812741
AGCGTCG245950.030.93377310
CTCGGTG245050.030.93010937
CGTGTAG243550.030.8325515
GTCGCCG239550.030.19667644
GTGTAGG254500.029.90457516
GAGCGTC257750.029.4555349
GCGTCGT253600.029.36194611
TGTAGGG263800.028.67909617
GATCTCG269900.028.62302434
TCGCCGT233600.028.5106745
CGTCGTG263350.028.33467712
GAGTGTA283700.027.71128727
ATCTCGG282100.027.43465835
AGAGCGT283050.027.1326858
GGTGGTC280750.026.26646240
GTCGTGT283400.026.18713413
Produced by FastQC (version 0.11.2)