FastQCFastQC Report
Wed 25 Feb 2015
lane2_Undetermined_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane2_Undetermined_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71364162
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC827040.1158901018132883TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG269350.031.60438211
ACACGTC278100.030.57845513
ACGTCTG279750.030.32655115
CACACGT284900.029.9425712
CACGTCT285550.029.74940114
CGTCTGA295700.028.86573216
GTCACGC157250.028.43134329
CAGTCAC305750.027.41181627
GAACTCC317100.026.52862421
TCACGCC167850.026.42245330
AGTCACG175400.026.27114528
AGCACAC326500.026.1687810
CCAGTCA322950.025.99350726
TCCAGTC332100.025.28411325
ACTCCAG343250.024.48091523
GTATGCC316350.024.47705345
CACGCCA183550.024.12562231
GATCGGA358650.024.1057191
CGTATGC320150.024.06693344
CTCGTAT326450.022.76323542
Produced by FastQC (version 0.11.2)