FastQCFastQC Report
Wed 25 Feb 2015
VE06_GGACTCCT_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameVE06_GGACTCCT_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5281160
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGC10100.012.2524348
TATACCG5500.011.8635965
GTCTAGC5100.011.470551
GTGTAGC6500.011.42303851
GTATACA15850.010.9305621
GCTAGAC5050.010.6930333
TAGACAG7600.010.6578595
ACCGTGC6150.010.609728
GTGTAGG9150.010.5737341
CTGGTAC6800.010.25731853
TAGACTG7950.010.1886455
ACTGTGC9950.010.1758458
GTGTTAG8250.010.0908751
TACACTC12000.09.9374675
GTCTAAG6850.09.8539811
GTGTAGA14550.09.7422361
GTGTACA13650.09.7252421
GCTCTAG6300.09.6428241
TACACTG13100.09.6182885
CTAGACA8550.09.4736524
Produced by FastQC (version 0.11.2)